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These codes for analysis singleCell/spatia/bulk RNA sequencing of ESCC in this paper: Jing Song, Xiaomei Song, Yue Xie, Hong Guo*, Yupeng Cun*. MIF-expressing tumor cells mediate immunotherapeutic resistance in esophageal squamous cell carcinoma. Theranostics 2026; 16(3):1613-1629. doi:10.7150/thno.118269